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Spectrophotometric method applicable to in vitro studies of Heinz body formation in erythrocytes


, : Spectrophotometric method applicable to in vitro studies of Heinz body formation in erythrocytes. Acta Haematologica 61(4): 222-225

A simple quantitative procedure was described for the estimation of Heinz body formation in [human] erythrocytes, based on the spectrophotometric measurement of the amount of crystal violet absorbed by precipitated Heinz bodies. Good correlation was shown between this method and the counting of Heinz body-containing erythrocytes in a microscopical preparation. This technique is particularly applicable to in vitro studies of the comparative effects produced in the erythrocyte by oxidant agents.

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Accession: 006460690

PMID: 108903

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Related references

Kunzer, W.; Schutz, E., 1955: Studies on Heinz body formation in the erythrocytes of the newborn. IV. Sodium nitrite and Heinz body formation. Zeitschrift für Kinderheilkunde 77(1): 101-106

Kunzer, W.; Schutz, E., 1956: Heinz body formation in erythrocytes in newborn infants. V. Heinz body formation in hemoglobin-free achromic erythrocytes. Zeitschrift für Kinderheilkunde 78(1): 96-103

Kleihauer, E.; Bernau, A.; Betke, K.; Keller, M.; Kohne, E.; Riegel, K., 1970: Heinz body formation in erythrocytes of the newborn. I. In vitro studies on experimental conditions and on the effect of exchange transfusions. Acta Haematologica 43(6): 333-347

Kleihauer E.; Bernau A.; Betke K.; Keller M.; Kohne E.; Riegel K., 1970: Heinz body formation in erythrocytes of new borns part 1 in vitro studies on experimental conditions and the effect of exchange transfusions. Acta Haematologica (Basel) 43(6): 333-347

Kuenzer, W.; Schuetz, E.; Bensch, J., 1960: Studies on Heinz body formation in erythrocytes in newborn infants. Part VI. On the morphology of Heinz bodies produced by analogues with vitamin K effect and vitamin C. Zeitschrift für Kinderheilkunde 83: 471-475

Faulhaber, H.D., 1962: The behavior of glutathione in erythrocytes in Heinz body formation. Experiments with dinitroglycol in vitro. Folia Haematologica 78: 672-675

Figge, F.H.J., 2014: Studies on the nature and significance of Heinz-body formation in erythrocytes. Anatomical Record 94: 461-461

Ohno, Y.I.; Nishikori, M.; Kanoh, T.; Uchino, H., 1982: Latent oxidative damage to erythrocytes and Heinz body formation in vitro: a study of an acetophenetidin abuser. A small intake of acetophenetidin (1.2 g for 7 days) induced hemolytic attack with Heinz bodies in RBC [red blood cells] and splenomegaly in a patient who was an intermittent drug abuser. The direct antiglobulin test was negative. Glucose-6-phosph...

Tjoa, G.T., 1960: Studies on Heinz body formation and glutathione stability in the erythrocytes of newborn infants. Zeitschrift für Kinderheilkunde 84: 484-488

Hopkins, J.; Tudhope, G.R., 1974: The effects of drugs on erythrocytes in vitro: Heinz body formation, glutathione peroxidase inhibition and changes in mechanical fragility. 1 The relationship between mechanical fragility, glutathione peroxidase inhibition and Heinz body formation, in erythrocytes exposed to oxidant drugs in vitro, has been investigated. All drugs tested caused Heinz body formation, and with the excep...