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Spectrophotometric method applicable to in vitro studies of Heinz body formation in erythrocytes


Acta Haematologica 61(4): 222-225
Spectrophotometric method applicable to in vitro studies of Heinz body formation in erythrocytes
A simple quantitative procedure was described for the estimation of Heinz body formation in [human] erythrocytes, based on the spectrophotometric measurement of the amount of crystal violet absorbed by precipitated Heinz bodies. Good correlation was shown between this method and the counting of Heinz body-containing erythrocytes in a microscopical preparation. This technique is particularly applicable to in vitro studies of the comparative effects produced in the erythrocyte by oxidant agents.


Accession: 006460690

PMID: 108903



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