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Spectrophotometric method for assaying 2 keto l gulonic acid


, : Spectrophotometric method for assaying 2 keto l gulonic acid. Prikladnaya Biokhimiya i Mikrobiologiya 13(3): 465-469

A spectrophotometric method for assaying 2-keto-L-gulonic acid based on measurements of the intensity of light absorption at .lambda. [wavelength] = 348 nm by the product of the acid reaction with orthophenyl diamine dissolved in acetic acid was described. The method can be used to determine 2-keto-L-gulonic acid in the culture [Pseudomonas] liquid and crystalline preparations. The relative error of measurements of 2-keto-L-gulonic acid is .+-. 2.38%.

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