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Spectrophotometric method for the estimation of arginine decarboxylase ec

, : Spectrophotometric method for the estimation of arginine decarboxylase ec Analytical Biochemistry 92(2): 331-337

A sensitive method for the determination of arginine decarboxylase [EC] from oat [Avena sativa] seedlings was developed, which is based upon the estimation of agmatine, the decarboxylation product of arginine. In the presence of pea [Pisum sativum] seedling amine oxidase [EC], the agmatine is oxidized and the H2O2 generated is estimated as a red-brown chromogen formed on the peroxidative oxidation of guaiacol. The method may be applied to the estimation of other amino acid decarboxylases.

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