+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Spectrophotometric screening method for acetaminophen in serum and plasma

Spectrophotometric screening method for acetaminophen in serum and plasma

Clinical Chemistry 26(1): 69-71

A simple, economical procedure for rapidly detecting acetaminophen, an analgesic antipyretic, in serum or plasma is described. The method is based on the reduction by the drug of ferric 2,4,6-tris(2-pyridyl)-s-triazine, at an acidic pH, to ferrous 2,4,6-tris(2-pyridyl)-S-triazine complex, which absorbs maximally at 593 nm. Absorbance and acetaminophen concentration are linearly related from 25-400 mg/l, and so therapeutic and toxic concentrations can be measured. The method is accurate; day-to-day CV [coefficient of variation] for 2 pooled control specimens (103 and 227 mg/l) were 4.4 and 6.6%. Correlation studies, with an established nitration method and with the free-radical diphenylpicrylhydrazyl dye method, showed correlation coefficients of 0.985 and 0.915, respectively. Of 25 commonly used drugs tested, only levodopa, oxyphenylbutazone and phenylephrine interfere significantly. Interference from salicylate, salicylamide and phenylbutazone was insignificant.

(PDF 0-2 workdays service: $29.90)

Accession: 006460747

Download citation: RISBibTeXText

PMID: 7356576

Related references

Rapid spectrophotometric method for the quantitation of acetaminophen in serum. Analytica Chimica Acta 443(1): 165-169, 12 September, 2001

Rapid spectrophotometric method for quantitation of acetaminophen in serum. Taiwan Yi Xue Hui Za Zhi. Journal of the Formosan Medical Association 84(6): 693-699, 1985

A convenient thin-layer chromatographic screening method for acetaminophen in serum. Journal of Analytical Toxicology 8(2): 54-58, 1984

Spectrophotometric method of estimating haemoglobin in plasma and serum. Arch. Sci. biol., Bologna. 37: 90-95, 1953

A spectrophotometric method for quantitating hemoglobin in plasma or serum. American Journal of Clinical Pathology 20(5): 429-433, 1950

A more sensitive spectrophotometric method for determination of isoniazid in serum or plasma. Clinical Chemistry 17(6): 492-494, 1971

A simple spectrophotometric method for quantitating hemoglobin in serum or plasma. J Amer Med Technol 28(1): 42-58, 1966

Acetaminophen in serum and plasma estimated by high-pressure liquid chromatography: a micro-scale method. Clinical Chemistry 23(4): 743-745, 1977

Spectrophotometric micro method for measuring cholin esterase activity in serum or plasma. Clinical Chemistry 18(5): 454-458, 1972

A new automatic spectrophotometric rate method for selective determination of glucose in serum, plasma, or blood. Anal. Chem, 34: 452-455, 1962

Elimination of interference by salicylate in spectrophotometric analysis of serum acetaminophen. Clinical Chemistry 27(6): 1104, 1981

A high-pressure liquid chromatographic method for the determination of N-acetyl-p-aminophenol (acetaminophen) in serum or plasma using a direct injection technique. Journal of Analytical Toxicology 5(1): 24-28, 1981

Specific spectrophotometric method for the determination of plasma and or serum hemoglobin using the 2 2 azino di 3 ethylbenzothiazoline 6 sulfonate chromogen. 1985

Optimization of spectrophotometric method for determination the concentration of catalytic activity Angiotensin Converting Enzyme in serum and plasma. Clinical Chemistry & Laboratory Medicine 37(SPEC SUPPL ): S469, June, 1999

A specific spectrophotometric method for determining plasma and/or serum hemoglobin. Use of the chromogen 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonate (ABTS). Annales de Biologie Clinique 43(3): 251-254, 1985