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Spectrophotometric study of guanylor rnase sa iii. circular dichroism spectra


Biologia (Bratislava) 41(4): 349-356
Spectrophotometric study of guanylor rnase sa iii. circular dichroism spectra
Conformational changes induced by changes of pH in guanyloribonuclease from Streptomyces aureofaciens RNase Sa were studied by circular dichroism. RNase Sa exhibited a complex negative circular dichroism around 280 nm with a clear fine structure and a positive Cotton effect at 235 nm. As circular dichroism around 280 nm is predominantly due to fixed tyrosine residues of RNase Sa, changes in their conformation and/or mobility were found to reflect clearly conformational changes of the enzyme. The protonization of tyrosines induced a negative Cotton effect at 240 nm, whereas after the ionization of tyrosines a positive band at 250 nm in circular dichroism spectra was observed. However, little conformational changes at pH 8.5 and weak conformational transition in the pH range from 5-3 were found. On the other hand, strong conformational changes at pH < 3 as well as pH > 10.5 were detected.

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Accession: 006460780



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