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Spectrophotometric study of influences on the direct ferric per chlorate method for the determination of serum cholesterol



Spectrophotometric study of influences on the direct ferric per chlorate method for the determination of serum cholesterol



Microchemical Journal 20(4): 428-439



A spectrophotometric study of several influences on a direct colorimetric determination of serum cholesterol is described. Interferences of in vitro and in vivo types were considered. Certain compounds, e.g., bromide, uracils and bilirubin, exerted both positive and negative interfering influences on the reaction of ferric perchlorate with cholesterol in an ethyl acetate-ethanol-sulfuric acid medium. Some interferences, e.g., bilirubin, are noncompeting side reactions which are absolute in their interference capabilities, while others, e.g., the uracils and bromide, have an entirely different influence. In the latter circumstance, the fine structures of spectra are altered by a nonadditive phenomenon of the reactant and hyperchromic (bromide) and hypochromic (uracils) effects take place which appear to result in relative rather than absolute errors.

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