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Spectroscopic studies on neurospora crassa copper metallo thionein


Biochemistry 22(9): 2043-2048
Spectroscopic studies on neurospora crassa copper metallo thionein
The spectral properties of Neurospora copper metallothionein were investigated and compared with those of the Cu(I)-2-mercaptoethanesulfonic acid complex. In both cases, the absorption spectra are rather similar, showing a characteristic shoulder at .apprx. 250 nm. Marked differences were observed in their emissive properties. Only metallothionein emits detectable luminescence in solution, but both the Cu protein and the Cu(I) complex are luminescent at 77.degree. K. The circular dichroism [CD] spectrum of Neurospora copper metallothionein shows several Cotton extrema attributable to asymmetry in metal coordination. The influence of HgCl2 and p-(chloromercuri)benzoate on the spectral properties of metallothionein was also investigated. The 2 mercurials exerted a pronounced effect on the electronic absorption, chiroptical and emissive properties of the protein. Spectroscopic titrations followed by gel filtration experiments indicate that 2 mercurials can be bound per metallothionein molecule without loss of Cu. This binding is responsible for the disappearance of the emissive properties of metallothionein and for the distinct changes in its electronic absorption and CD spectra. From these data, it is suggested that the Cu(I) ions are coordinated to the cysteinyl residues in the form of a single metal cluster.


Accession: 006461081



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