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Spectroscopic studies on the interactions of achatina hemocyanin with some halides

, : Spectroscopic studies on the interactions of achatina hemocyanin with some halides. Proceedings of the National Science Council Republic of China Part A Physical Science & Engineering 10(3): 206-211

The effect of potassium fluoride, chloride, and bromide on the oxygen binding of Achatina hemocyanin from Taiwan snail has been studied by ultraviolet spectrophotometric, fluorescence, and 19F NMR methods. The presence of KCl and KBr up to 0.8 M does not affect the binding of oxygen in oxyhemocyanin. Addition of KF at pH 5.3 causes a decrease in absorbance at 345 nm and an enhancement of the fluorescence yield of oxyhemocyanin, which are ascribed to the displacement of peroxide from the active site of hemocyanin by the neutral HF molecule. The apparent dissociation constant for the HF-hemocyanin complex was evaluated to be 0.22 M, and the primary reaction occurred with observed rate constants of 0.038 h-1 for 0.3 M, at pH 5.3. At pH 9.0 fluoride ion binds to oxyhemocyanin with the binding constant of 0.52 M-1 and does not affect the absorbance at 345 nm and the fluorescence yield.

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