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Speract binds exclusively to sperm tails and causes an electrophoretic mobility shift in a major sperm tail protein of sea urchins



Speract binds exclusively to sperm tails and causes an electrophoretic mobility shift in a major sperm tail protein of sea urchins



Zoological Science (Tokyo) 4(4): 641-648



Intact spermatozoa, sperm heads and sperm tails of the sea urchin Hemicentrotus pulcherrimus were incubated with various concentrations of synthetic speract. The results of bioassay of the residual respiration stimulation activity in the supernatant obtained from each incubation mixture showed that more than 99% of speract in the original solution bound to sperm tails as well as intact spermatozoa, but little to sperm heads. Speract caused a mobility change of a major high-molecular weight sperm tail protein in H. pulcherrimus or Anthocidaris crassispina on SDS-polyacrylamide gels.

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Accession: 006461602

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