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Sperm chromosome analysis as a new system to test human x sperm and y sperm separation

Sperm chromosome analysis as a new system to test human x sperm and y sperm separation

Gamete Research 17(3): 221-228

The correlation between the percentage of F-body-positive spermatozoa (F-sperm) and that of Y-chromosome-bearing spermatozoa (Y-sperm) determined by human sperm chromosome analysis was examined after spermatozoa were separated by an albumin column method [Ericsson et al, Nature 246:421-424:1973]. The percentages of F-sperm were 43.1.+-.4.8 (36.1-48.8) and 45.8.+-.8.3 (35.3-66.5) before and after separation, respectively. A slight but significant (p < 0.05) increase in F-sperm after the separation was observed in two semen donors. A total of 570 sperm karyotype was analyzed and the percentage of Y-sperm ranged from 36.0 to 59.1 with a mean of 47.3. In one semen donor, the percentage of Y-sperm (45.6) was significantly (p < 0.05) higher than that of F-sperm (35.3) after separation. In the remaining nine semen donors, the results obtained by chromosome analysis corresponded well with those obtained by F-body scoring. The validity of the albumin column method in isolating a Y sperm-rich sperm population is discussed.

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