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Sperm head de condensation by a high molecular weight fraction of sea urchin lytechinus variegatus egg homogenate


, : Sperm head de condensation by a high molecular weight fraction of sea urchin lytechinus variegatus egg homogenate. Journal of Experimental Zoology 212(2): 159-168

Sperm nucleus decondensing activity (NDA) of eggs was investigated using the gametes of the sea urchin, L. variegatus. To assay for NDA, isolated sperm heads were exposed to homogenate from mature unfertilized ova and then examined with phase-contrast optics. NDA is retained by dialysis and requires Ca2+ at the time of homogenization and at the time of assay. Other divalent cations (Mg2+, Sr2+, Ba2+, Mn2+) can substitute for Ca2+, but are much less effective. NDA is stable to cold (-20.degree. C, -76.degree. C), but not to heat (56.degree. C). The pH activity optimum is in the range 7-9. NDA is present in the supernatant after centrifugation at 150,000 g for 105 min. It elutes shortly following the void volume on a Sephacryl S-200 column, with an approximate MW of 100,000 daltons.

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Related references

Eng L.A., 1979: De condensation of sea urchin lytechinus variegatus sperm nuclei by egg homogenate. Biology of Reproduction 20(SUPPL 1): 52A

Eng, L.A.; Metz, C.B., 1980: Sperm head decondensation by a high molecular weight fraction of sea urchin egg homogenate. Sperm nucleus decondensing activity (NDA) of eggs has been investigated using the gametes of the sea urchin, Lytechinus variegatus. To assay for NDA, isolated sperm heads were exposed to homogenate from mature unfertilized ova and then examined wi...

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