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Sperm motility in the horseshoe crab 3. isolation and characterization of a sperm motility initiating peptide


, : Sperm motility in the horseshoe crab 3. isolation and characterization of a sperm motility initiating peptide. Gamete Research 6(4): 315-326

Sperm motility in Limulus is initiated by a sperm motility initiating factor (SMI) that emanates from Limulus eggs. The partial purification of SMI (> 230-fold purification with respect to protein content) with 40% recovery is reported. SMI appears to be a hydrophobic peptide of 500-2000 MW. Although probably not purified to homogeneity, SMI is estimated to be active at a concentration of < 0.2 .mu.M.

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Related references

Clapper D.L.; Epel D., 1981: Isolation and utilization of a sperm motility initiating peptide in the horseshoe crab limulus polyphemus evidence for involvement of calcium ii but not intra cellular ph or membrane potential in motility initiation. Journal of Cell Biology 91(2 PART 2): 179A

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