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Sperm motility in the horseshoe crab 3. isolation and characterization of a sperm motility initiating peptide

, : Sperm motility in the horseshoe crab 3. isolation and characterization of a sperm motility initiating peptide. Gamete Research 6(4): 315-326

Sperm motility in Limulus is initiated by a sperm motility initiating factor (SMI) that emanates from Limulus eggs. The partial purification of SMI (> 230-fold purification with respect to protein content) with 40% recovery is reported. SMI appears to be a hydrophobic peptide of 500-2000 MW. Although probably not purified to homogeneity, SMI is estimated to be active at a concentration of < 0.2 .mu.M.

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