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Sperm penetration assay: useful test in evaluation of male fertility


, : Sperm penetration assay: useful test in evaluation of male fertility. Obstetrics and Gynecology 57(5): 620-623

The recently developed sperm penetration assay (SPA) tests the functional capacity of sperm. It is based upon the ability of in vitro capacitated human spermatozoa to penetrate zona-free hamster eggs in vitro. The SPA was superior to seminal fluid analysis in predicting both fertility and infertility; in the prediction of infertility, the difference was highly significant. Data further indicated that fertility in the male may not remain constant with the passage of time. The SPA would appear to have wide application both clinically and experimentally.

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Accession: 006461834

PMID: 7219910

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Related references

Leroy Martin B.; Saint Pol P.; Bouhdida M.; Hermand E., 1989: The correlation between the zona free hamster egg penetration assay and the other parameters of the sperm the value of this test in investigating male fertility. On investigating infertility in 162 couples the zona-free hamster egg penetration test was carried out. As a control we tested the ejaculates of 34 fertile men. There is a significant correlation between the percentage of oocytes that were penetra...

Ahmadi, A.; Ng, S.C.ye, 1999: Destruction of protamine in human sperm inhibits sperm binding and penetration in the zona-free hamster penetration test but increases sperm head decondensation and male pronuclear formation in the hamster-ICSI assay. Purpose: Our purpose was to investigate the fertilizing ability of human protamine-damaged sperm in a heterologous system using hamster oocytes. Methods: The protamine of the sperm were damaged by exposure to dithiothreitol, a disulfide-reducing a...

Ahmadi, A.; Ng, S.C., 1999: Destruction of protamine in human sperm inhibits sperm binding and penetration in the zona-free hamster penetration test but increases sperm head decondensation and male pronuclear formation in the hamster-ICSI assay. Our purpose was to investigate the fertilizing ability of human protamine-damaged sperm in a heterologous system using hamster oocytes. The protamine of the sperm were damaged by exposure to dithiothreitol, a disulfide-reducing agent. Their abilit...

Peter J., 1985: Sperm penetration assay assessment of male fertility. Journal Of Andrologyuppl: 138-P

Lazarevic, J.; Wikarczuk, M.; Somkuti, S.G.; Barmat, L.I.; Schinfeld, J.S.; Smith, S.E., 2010: Hyaluronan binding assay (HBA) vs. sperm penetration assay (SPA): Can HBA replace the SPA test in male partner screening before in vitro fertilization?. To determine if a less expensive, easier, and faster to perform HBA test is clinically equal to the more complicated, technically challenging and expensive SPA test as a reliable indicator of sperm fertilizing capacity. Prospective study. Androlog...

Hensleigh, HC.; Byers, AP.; Post, GS.; Reindl, N.; Seal, US.; Tilson, RL., 1987: Evaluation of tiger fertility by the sperm penetration assay. Unknown

Francavilla, F.; Romano, R.; Santucci, R.; Marrone, V.; Properzi, G.; Ruvolo, G., 1997: Occurrence of the interference of sperm-associated antibodies on sperm fertilizing ability as evaluated by the sperm-zona pellucida binding test and by the TEST-yolk buffer enhanced sperm penetration assay. This study was performed to evaluate the occurrence as well as the level of the interference of sperm-associated antibodies on fertilization process. Motile sperm suspensions from 28 infertile patients with high degree of autoimmunization against...

Santucci, R.; Romano, R.; Marrone, V.; Properzi, G.; Ruvolo, G.; Francavilla, F., 1997: Occurrence of the interference of sperm-associated antibodies on sperm fertilizing ability as evaluated by the sperm-zona pellucida binding test and by the TEST-yolk buffer enhanced sperm penetration assay. PROBLEM: This study was performed to evaluate the occurrence as well as the level of the interference of sperm-associated antibodies on fertilization process. METHOD: Motile sperm suspensions from 28 infertile patients with high degree of autoimmu...

Fukuda M.; IIda M.; Takeuchi H.; Takada M., 1991: Sperm penetration assay in evaluation for male infertility. An analysis of semen quality was carried out in 27 fertile donors and the male partners of 49 infertile couples by the conventional semen analysis and the sperm penetration assay (SPA). Sperm motility and progression showed a significant relations...

Kawashima, K.; Ono, A., 1997: Fundamental examination on application of the zona free hamster egg sperm penetration test for male rat fertility testing. Congenital Anomalies 37(3): 310, Sept