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Stepwise oligo nucleotide synthesis 28. the immobilized rnase of penicillium brevicompactum in oligo ribo nucleotide synthesis



Stepwise oligo nucleotide synthesis 28. the immobilized rnase of penicillium brevicompactum in oligo ribo nucleotide synthesis



Bioorganicheskaya Khimiya 6(5): 736-742



The P. brevicompactum RNase manifesting a low specificity was covalently bound to CM-cellulose for the purpose of a preparative oligoribonucleotide synthesis. The hydrolytic and synthetic activities of immobilized RNAse are very similar to those of the native enzyme and are not lost on repeated use of the preparation in the hydrolysis of synthesis (to about 10 times). The immobilization does not affect the substrate specificity but alters the pH optimum of the enzyme for some substrates: pH optimum shifts from 4.6 to 7.0 for the synthesis of GpC. The obtained preparation is effective catalyst of the oligoribonucleotide synthesis.

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