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Chapter 6,479

Sterility in successive backcrosses of raphanobrassica 2n equals 4x equals 36 with recurrent brassica oleracea 2n equals 2x equals 18

Mccollum, G.D.

Canadian Journal of Genetics and Cytology 21(4): 479-486

1979

Accession: 006478565
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A series of crosses to develop cytoplasmic male sterility in the cole crops by transferring the B. oleracea L. (2n = 18) genome into the cytoplasm of radish (Raphanus sativus L., 2n = 18) through the amphidiploid Raphanobrassica (2n = 4x = 36) was terminated because of complete seed sterility of the only 2 BC6 [backcross] hybrid plants obtained. There was no improvement of seed set in 6 backcrosses to diploid B. oleracea, and the chromosome numbers of the few plants producing seeds were higher than expected, frequently being close to the triploid number (2n = 3x = 27) of the BC1. Zygotes with < 2 radish chromosomes were not found; those with < 4 were few; and those with < 6 rarely produced offspring.

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