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Structural proteins of type c rna leukemia viruses and auto immune diseases of new zealand mice biochemical analysis of murine leukemia virus structural proteins in the serum and murine leukemia virus cell surface antigens detectable by endogenous antibody



Structural proteins of type c rna leukemia viruses and auto immune diseases of new zealand mice biochemical analysis of murine leukemia virus structural proteins in the serum and murine leukemia virus cell surface antigens detectable by endogenous antibody



Virus 28(2): 103-111



Gp70-related molecules in the serum were heavily deposited in the lupus kidney and vascular lesions as an immune complex in New Zealand mice. They were characterized biochemically by using affinity chromatography and SDS-PAGE [sodium dodecyl sulfate-polyacrylamide gel electrophoresis] analysis. Isolated gp70-related molecules in the serum consisted of 3 glycoprotein fragments with an approximate MW of 53,000, 45,000 and 32,000 daltons, respectively. Gp70 isolated from the cell surface affected with mouse leukemia E.male.G2 was a single glycoprotein molecule with a MW of approximately 70,000 daltons. The major gp70 activity in the serum fractionated by Sephadex gel filtration was of MW ranging 120,000-140,000. The minor gp70 activity was of the same size as serum albumin. The 3 glycoprotein fragments of serum gp70 possibly resulted from specific transcriptional, translational or post-translational process or from non-specific proteolysis at susceptible sites. A gp70-related molecule in the serum may be a specific envelope-related glycoprotein which consists of 3 glycoprotein fragments with a MW of 53,000, 45,000 and 32,000 daltons, respectively. Serum gp70 may be associated with host cellular components or present as a dimer. Pooled B/WF1 mouse sera from 2 age groups were fractionated by Sephadex gel filtration. The major gp70 activity of 2 mo. old B/WF1 serum was found in fractions with a MW in a range of 120,000-140,000 daltons. The gp70 activity of 9 mo. old B/WF1 serum eluted for the most part at the void volume. The 125I-labeled gp70-anti-gp70 immune complex prepared in vitro eluted at the void volume. gp70-Related molecules in the serum of aged B/WF1 mice apparently were present largely as immune complexes. To identify directly the molecule(s) bearing the antigenic determinants for endogenous antibody to MuLV [murine leukemia virus] or Gross natural antibody (GNA), 125I-E.male.G2 MuLV proteins were subjected to immunoprecipitation with GNA-positive mouse sera. SDS-PAGE analysis of the resulting precipitates revealed that the major molecule bearing antigenic determinants for GNA is p(85), the precursor polyprotein of the MuLV core structural proteins, p30, p15 and p10.

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