Structure and lysis of micro sclerotia of verticillium dahliae in soil
Baard, S.W.; Van Wyk, P.W.J.; Pauer, G.D.C.
Transactions of the British Mycological Society 77(2): 251-260
Microsclerotia of V. dahliae placed in soils of high and low pH quickly became colonized by bacteria. Fungal colonization was also observed in both soil types. Bacterial colonization was more prevalent in soil of high pH while fungal colonization was most evident in soil of low pH. Various bacteria seemed to be involved in the colonization of the microsclerotia. Some were attached to the microsclerotial cell walls and seemed to cause a swelling of the walls; others appeared embedded in the remains of cell walls which had lost their electron-dense structure and apparently were lysed. Bacterial also penetrated microsclerotial cells through pores in the cell walls. Fungal penetration of microsclerotial cell walls was observed less frequently, but fungal structures were observed in and around microsclerotial cells. Microsclerotia incubated in soil for 3 mo. showed considerable loss of electron-dense material situated within the matrix between the cells which apparently indicated deterioration of pigmented material.