+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Structure of eukaryotic chromosomes and chromatin part 1 reconstituted histone dna complexes



Structure of eukaryotic chromosomes and chromatin part 1 reconstituted histone dna complexes



Philosophical Transactions of the Royal Society of London B Biological Sciences 283(997): 259-268



Complexes of [phage] .lambda.dv 21 plasmid DNA with calf thymus histones are reconstituted in the presence and absence of urea and, in the absence of urea, at 4 and 37.degree. C. The preferential binding of individual histones to certain DNA sequences is abolished, by specific histone-histone interactions, when a mixture of the 4 small histones is used (Steinmetz, Streeck and Zachau). This also holds for the other conditions tested. An exception is the pair of arginine-rich histones (H3-H4) which appears to bind specifically when reconstituted in the absence of urea by a 1 step dialysis procedure, and non-specifically when the salt is removed by a stepwise dialysis procedure. The subunits which are formed when the 4 small histones are present simultaneously are very similar to nucleosome core particles according to the fragment patterns obtained with micrococcal nuclease. Protection of 155, 285, 420, 560, etc. nucleotide pairs is observed. When histone H1 is present in addition to the 4 small histones, a 175 nucleotide pair fragment is protected, but no 200 nucleotide pair fragment or multiples were formed under these conditions.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 006496503

Download citation: RISBibTeXText


Related references

Structure of Eukaryotic Chromosomes and Chromatin || Reconstituted Histone-DNA Complexes. Philosophical Transactions of the Royal Society of London Series B Biological Sciences 283(997): 259-268, 1978

Structure of eukaryotic chromosomes and chromatin part 1 histone interactions histone modifications and chromatin structure. Philosophical Transactions of the Royal Society of London B Biological Sciences 283(997): 291-294, 1978

Structure of eukaryotic chromosomes and chromatin part 2 aspects of the regulation of histone genes. Philosophical Transactions of the Royal Society of London B Biological Sciences 283(997): 319-324, 1978

Structure of Eukaryotic Chromosomes and Chromatin || Histone Interactions, Histone Modifications and Chromatin Structure. Philosophical Transactions of the Royal Society of London Series B Biological Sciences 283(997): 291-293, 1978

Structure of eukaryotic chromosomes and chromatin part 1 conformational flexibility in dna structure and its implications in understanding the organization of dna in chromatin/. Philosophical Transactions of the Royal Society of London B Biological Sciences 283(997): 295-298, 1978

Structure of Eukaryotic Chromosomes and Chromatin || Aspects of the Regulation of Histone Genes. Philosophical Transactions of the Royal Society of London Series B Biological Sciences 283(997): 319-324, 1978

Structure of eukaryotic chromosomes and chromatin part 3 organization of transcribed regions of chromatin. Philosophical Transactions of the Royal Society of London B Biological Sciences 283(997): 343-358, 1978

Structure of eukaryotic chromosomes and chromatin part 3 chromatin transcription with mercurated nucleotides. Philosophical Transactions of the Royal Society of London B Biological Sciences 283(997): 379-380, 1978

Structure of eukaryotic chromosomes and chromatin part 4 molecular architecture of drosophila melanogaster chromatin. Philosophical Transactions of the Royal Society of London B Biological Sciences 283(997): 407-410, 1978

Structure of eukaryotic chromosomes and chromatin part 4 higher order structure of mitotic chromosomes. Philosophical Transactions of the Royal Society of London B Biological Sciences 283(997): 415-416, 1978

Structure of eukaryotic chromosomes and chromatin part 3 the organization of transcription on lampbrush chromosomes. Philosophical Transactions of the Royal Society of London B Biological Sciences 283(997): 359-366, 1978

Structure of eukaryotic chromosomes and chromatin part 2 some trends in the evolution of very large chromosomes. Philosophical Transactions of the Royal Society of London B Biological Sciences 283(997): 309-318, 1978

Structure of eukaryotic chromosomes and chromatin part 1 on the structure of cellular and viral chromatin. Philosophical Transactions of the Royal Society of London B Biological Sciences 283(997): 274-286, 1978

Structure of eukaryotic chromosomes and chromatin part 1 the structure of sv 40 chromatin. Philosophical Transactions of the Royal Society of London B Biological Sciences 283(997): 299-304, 1978

Structure of eukaryotic chromosomes and chromatin part 3 introductory remarks. Philosophical Transactions of the Royal Society of London B Biological Sciences 283(997): 331-332, 1978