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Studies of isoniazid metabolism in isolated rat hepatocytes by mass fragmentography


Studies of isoniazid metabolism in isolated rat hepatocytes by mass fragmentography



Journal of Chromatography 230(2): 345-352



ISSN/ISBN: 0021-9673

PMID: 7107780

DOI: 10.1016/s0378-4347(00)80484-3

Isoniazid metabolism in isolated rat hepatocytes was studied by mass fragmentography, using single ion monitoring. Isoniazid and its metabolites were determined as the trimethylsilylated derivatives of acetylisoniazid and diacetylhydrazine and of the benzaldehyde hydrazones of isoniazid and acetylhydrazine. Deuterated analogs served as internal standards. Hydrazine was quantitated as benzalazine using 15N-labeled hydrazine as an internal standard. The method is well suited for the microanalysis of isoniazid metabolites in specificity and reliability to demonstrate the overall pathway of isoniazid metabolism, from which it was clarified that the greater part of hydrazine, a hazardous metabolite of isoniazid, was formed through the direct hydrolysis of isoniazid itself as expected.

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Accession: 006501420

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