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Studies of the immuno globulin eluted from the glomeruli of mice chronically infected with lymphocytic choriomeningitis virus



Studies of the immuno globulin eluted from the glomeruli of mice chronically infected with lymphocytic choriomeningitis virus



Journal of Immunology 119(2): 707-713



Previous work demonstrated that B [bone marrow-derived] cells in the lymphocytic choriomeningitis virus (LCM) carrier mouse do not produce anti-LCM antibody. Studies were undertaken to determine the binding specificity of immunoglobulin (Ig) eluted from kidneys of LCM-infected SWR/J mice. Kidneys from neonatally infected LCM carrier mice (5-7 mo. of age) were homogenized in 0.05 M EDTA PBS [phosphate buffered saline] (pH 7.4), centrifuged at 12,000 .times. g for 10 min at 4.degree. C, and the sediment was washed 3 times. The sediment was eluted with citrate buffer (pH 3.2) for 90 min at 37.degree. C, centrifuged as before, neutralized and concentrated by vacuum dialysis. These elution conditions destroyed the binding capacity of LCM antigen in infected tissues and in commercial complement fixation LCM antigen solutions. The amount of Ig eluted was 22-24 .mu.g/kidney from LCM-infected mice but < 1 .mu.g/kidney from uninfected mice. Eluted Ig did not bind to LCM-infected tissue although SWR/J anti-LCM antiserum subjected to the eluting conditions retained its LCM antigen-binding ability. NZB .times. NZW F1 hybrid kidneys eluted as above yielded similar amounts of Ig that had anti-nuclear binding ability. Eluted Ig did not induce complement fixation when added to LCM antigen, although SWR/J anti-LCM antiserum subjected to elution conditions retained its LCM antigen complement-fixing properties. Ig deposited in glomeruli of LCM carrier mice is probably not specific for LCM antigens. The LCM carrier mouse is probably tolerant to LCM virus.

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Accession: 006502776

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PMID: 889613


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