Studies on body surface mucin of a fish norogenge allolepis hollandi part 1 chemical compositions of glyco proteins and glyco peptide/
Hashimoto, H.; Yoshimura, J.
Nippon Suisan Gakkaishi 43(11): 1319-1326
1977
ISSN/ISBN: 0021-5392 Accession: 006505015
Several glycoproteins were isolated under neutral conditions from the body-surface mucin of a fish, "Norogenge" (Allolepis hollandi), purified and their chemical compositions examined. Pronase digestion of these glycoproteins gave 2 kinds of glycopeptides which were separated by DEAE-cellulose column chromatography. The 1st and major component contained glucosamine, neutral sugars (galactose and mannose), N-acetylneuraminic acid and aspartic acid in molar ratios of 3:2:6-7:1. This glycopeptide contained some other amino acids as well. The linkage between carbohydrate and protein was deduced to be of the N-glycosidic type between asparagine and glucosamine.