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Studies on enterotoxin plasmids. III. Analysis of plasmids in resistant and enterotoxigenic Escherichia coli by gel electrophoresis

Studies on enterotoxin plasmids. III. Analysis of plasmids in resistant and enterotoxigenic Escherichia coli by gel electrophoresis

Wei Sheng Wu Xue Bao 25(3): 245-249

The results, based on the surveys of drug resistance, transconjugation, gel electrophoresis of plasmid DNA and enterotoxin production of 20 strains of enterotoxigenic and drug resistant E. coli of Human Origin, indicated the naturally occurring plasmid carrying genes for enterotoxin production and drug resistance has not yet been found in our province. Under the antibiotic selective pressure, R and Ent plasmids can be co-transferred from eight of the twelve enterotoxigenic and multi-resistant strains to their recipients. Thus the recipients acquired enterotoxigenicity and drug-resistance similar to the donors, and showed the same two large plasmid bands as the donors by gel electrophoresis. The larger one was R plasmid and the smaller one was Ent plasmid with its molecular weight corresponding to F plasmid. We found a transconjugant (7930/C600 No. 3) which showed by gel electrophoresis only a single plasmid band larger than Ent plasmid for several times. But this conjugant had the same enterotoxigenicity and drug resistance as the donor. After continuous cultivation and conjugation to another recipient, the above mentioned three properties were kept unchanged. So we suppose the plasmid band larger than Ent plasmid to be and Ent-R recombinant by transposition. Both the co-transfer and the recombination of R and Ent plasmids will bring more difficulties to prevent and treat diarrhea, hence special attention should be paid to this problem.

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Accession: 006506768

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PMID: 3915626

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