Studies on filariasis v. exsheathment of brugia pahangi and brugia malayi microfilariae in the mosquitoes aedes aegypti aedes togoi culex pipiens molestus and armigeres subalbatus
Ogura, N.; Kobayashi, M.; Tsuruoka, H.; Chigusa, Y.; Yamamoto, H.
Japanese Journal of Parasitology 35(2): 75-82
1986
Accession: 006507128
The present paper describes some experiments with exsheathment of Brugia pahangi and B. malayi microfilariae in the abdominal haemocoel of the mosquitoes, Aedes aegypti, Ae. togoi, Culex pipiens molestus and Armigeres subalbatus. Sheathed larvae were recovered from the abdominal haemocoel of Ae. aegypti and Ae. togoi fed on the jirds infected with B. pahangi. Sheathed larvae of B. pahangi were also found in the medium where the mid-gut of Ae. aegypti and Ae. togoi had been incubated for 2 hrs. Approximately 40% of the ingested larvae were supposed to penetrate through the mid-gut wall to the haemocoel without casting off their sheath when mosquitoes had been fed on the donors of relatively low microfilaria density (2.6 mf/cmm). About 20% or more of microfilariae which were collected from the donor's blood and injected into the abdominal haemocoel of Ae. aegypti, Ae. togoi and Cx. pipiens molestus were able to develop to the 3rd stage larvae. In the experiments of B. malayi, sheathed larvae also observed in the abdominal haemocoel of Ae. aegypti, Ae. togoi and Ar. subalbatus. These results suggests that exsheathment of the sheathed larvae takes place commonly in the haemocoel as well as in the mid-gut and that they are able to complete their development to the 3rd stage larvae.