Studies on infection and multiplication of plant viruses in variegated tobacco nicotiana tabacum cultivar samsun nc plants 1. the multiplication of cucumber mosaic virus in white and green areas of variegated leaves

Nagao, N.

Science Bulletin of the Faculty of Agriculture Kyushu University 35(3-4): 121-128


Accession: 006508494

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Cucumber mosaic virus (CMV) multiplication in white (W) and green (G) areas of variegated leaves of tobacco plants (N. tabacum cv. Samsun Nc) was studied to understand the relationship between virus multiplication and the chloroplast. The infectivity of extracts from W and G was first detected at 24 h after inoculation and increased with time almost in parallel on both areas, reached peaks at 5 days and then decreased. Virus concentration in W was always 1.7-3.9 times higher than that in G. RNase activity in healthy leaves and inhibitory effect of CMV-excluded-supernatant-solution prepared from the inoculated leaves on CMV infectivity were higher in W than in G. The virus movement from the inoculated G to opposite uninoculated W was detected 6 and 4 days after inoculation in attached and detached leaves, respectively. The virus movement from G to G was retarded 1-3 days longer than that from G to W. EM observation of W and G from leaf tissues 4 days after inoculation revealed that CMV particles were not in the form of crystalline arrays or large masses but scattered with ribosomes in the cytoplasm of mesophyll tissue. The difference in CMV concentration between W and G is caused mainly by the differential photosynthetic ability which affects the sensitivity of host tissues to virus infection and multiplication.