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Studies on nucleic acid metabolism in rats using carbon 14 labeled purine and pyrimidine bases and nucleic acids part 1 catabolism of nucleic acid derivatives



Studies on nucleic acid metabolism in rats using carbon 14 labeled purine and pyrimidine bases and nucleic acids part 1 catabolism of nucleic acid derivatives



Zeitschrift fuer Tierphysiologie Tierernaehrung und Futtermittelkunde 40(5): 236-247



(8-14C)-Labeled purine bases adenine and guanine, and (2-14C) labeled pyrimidine bases cytosine, uracil and thymine were administered to rats either i.v. or orally. (U-14C) nucleic acids were administered orally. After 24 h, the excretion of 14C-activity in respired air, urine and feces was determined. After i.v. injection of (8-14C) adenine, renal excretion of 14C-activity was also followed for a period of 21 days. Significant amounts of activity were excreted via feces only after oral administration. The rate of absorption was higher for nucleic acids (94%) than for the free bases adenine and cytosine (86%). After administering purine bases and cytosine, radioactivity was excreted mainly in the urine; after administering uracil and thymine or nucleic acids, the activity was present mainly as respiratory 14CO2. Uracil and thymine produced almost identical curves of 14CO2, regardless of the mode of administration. 14CO2-activity was higher after oral administration of adenine, guanine and especially cytosine than after i.v. injection. With the exception of i.v. injected (8-14C) adenine and orally administered (U-14C) nucleic acids, the substances were catabolized almost completely. About 70% of the 14C-activity ingested orally as adenine was excreted in the urine within 24 h; after i.v. injection, this level was not reached for 21 days. The different metabolic fates of orally or i.v. administered adenine and cytosine suggest that microorganisms contribute to the breakdown of these substances.

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