EurekaMag
+ Translate
+ Most Popular
Gaucher's disease;thirty-two years experience at Siriraj Hospital
A study of Macrobathra Meyrick from China (Lepidoptera, Cosmopterigidae)
First occurrence in ores of tetragonal chalcocite
Effects of trace element nutrition on sleep patterns in adult women
N.Z. range management guidelines. 2. Design of grazing management systems for tussock country
A case of lipoma of the esophagus
A revision of world Acanthosomatidae (Heteroptera: Pentatomidae): keys to and descriptions of subfamilies, tribes and genera, with designation of types
Life history of the coronate scyphozoan Linuche unguiculata (Swartz, 1788)
Perceptual restoration of obliterated sounds
Mutagenicity studies on two chromium(III) coordination compounds
The formation of the skeleton. I. Growth of a long bone. 1st appearance of a center of calcification
Leucopenia and abnormal liver function in travellers on malaria chemoprophylaxis
The joint commission: four key root causes loom large in sentinel event data
Treatment of vitiligo with topical 15% lactic acid solution in combination with ultra violet-A
Behaviour of dairy cows within three hours after feed supply: I. Influence of housing type and time elapsing after feed supply
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Management and control of patients with type 2 diabetes mellitus in Lebanon: results from the International Diabetes Management Practices Study (IDMPS)
The diet composition and nutritional knowledge of patients with anorexia nervosa
Physoporella croatica Herak, 1958 of the Slovak karst Anisian (Slovakia, the West Carpathians Mts.)
Bright lights, big noise. How effective are vehicle warning systems?
Ein Plesiosaurier-Rest mit Magensteinen aus mittlerem Lias von Quedlinburg
Incidence of Chlamydia trachomatis in patients with sterility
Monster soup: the microscope and Victorian fantasy
Preliminary tests with residual sprays against poultry lice
Duration of the life of plants in phylogeny

Studies on perivitelline fluid of horseshoe crab embryo. I. Purification and properties of agglutinin from the perivitelline fluid of Tachypleus gigas embryo


Studies on perivitelline fluid of horseshoe crab embryo. I. Purification and properties of agglutinin from the perivitelline fluid of Tachypleus gigas embryo



Journal of Biochemistry 96(3): 621-628



ISSN/ISBN: 0021-924X

PMID: 6542100

Agglutinin in the perivitelline fluid (PVF) of T. gigas, horseshoe crab, embryo was isolated and purified by a combination of affinity column chromatography on Sepharose 4B coupled with bovine submaxillary gland mucin and gel-filtration of Fractogel TSK (Toyopearl) HW-60 in Tris-NaCl-CaCl2 (0.05 M Tris-HCl, pH 7.5, containing 0.5 M NaCl and 0.1 M CaCl2) buffer containing 1 M urea. The specific activity of the purified protein was increased .apprx. 1300 times in comparison with that of the starting material. The active protein was present in highly polymerized forms which were multimers of an identical subunit with a MW of .apprx. 40,000 as measured by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. This agglutinin was shown to have multimeric activity towards different kinds of erythrocytes and its hemagglutinating activity was inhibited by N-acetylamino sugars and bovine submaxillary gland mucin containing sialic acid. Urea and guanidine-HCl inhibited the agglutinating activity but the activity recovered after dilution or dialysis, whereas the effect of HCl, NaOH or 2-mercaptoethanol was irreversible.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 006510665

Download citation: RISBibTeXText

Related references

Studies on perivitelline fluid of horseshoe crab embryo. II. Purification of agglutinin-binding substance from the perivitelline fluid of Tachypleus gigas embryo. Journal of Biochemistry 96(3): 629-636, 1984

Studies on Perivitelline Fluid of Horseshoe Crab Embryo. Ii. Purification of Agglutinin-Binding Substance from the Perivitelline Fluid of Tachypleus gigas Embryo12. The Journal of Biochemistry 96(3): 629-636, 1984

Protein components in the perivitelline fluid of the embryo of the horseshoe crab,Tachypleus tridentatus. Developmental Biology 73(2): 183-192, 1979

Enhancement of vertebrate cardiogenesis by a lectin from perivitelline fluid of horseshoe crab embryo. Cellular and Molecular Life Sciences: Cmls 65(20): 3312-3324, 2008

Changes in Osmotic Pressure and Swelling in Horseshoe Crab Embryos During Development : (horseshoe crab embryo/swelling/water influx/perivitelline fluid/osmotic pressure). Development, Growth and Differentiation 27(1): 51-56, 1985

Purification, characterization, and amino acid sequence of an embryonic lectin in perivitelline fluid of the horseshoe crab. Journal of Biological Chemistry 274(53): 37673-37678, 1999

Protein components in the perivitelline fluid of the embryo of the horseshoe crab, Tachypleus tridentatus. Developmental Biology 73(2): 183-192, 1979

Enhancement of angiogenesis by a 27 kDa lectin from perivitelline fluid of horseshoe crab embryos through upregulation of VEGF and its receptor. Journal of Natural Products 76(1): 117-120, 2013

Effects of Perivitelline Fluid Obtained from Horseshoe Crab on The Proliferation and Genotoxicity of Dental Pulp Stem Cells. Cell Journal 17(2): 253-263, 2015

The chemical properties of the perivitelline fluid of the fourth embryonic molt embryo and hatching stage trilobite larva of limulus polyphemus. Journal of Cellular Biochemistry Suppl (11 Part C): 17, 1987

Studies on the embryo development of horseshoe crab, Tachypleus tridentatus. Journal of Xiamen University Natural Science ; 41(2): 239-246, 2002

Agglutinins in the horseshoe crab hemolymph: purification of a potent agglutinin of horse erythrocytes from the hemolymph of Tachypleus tridentatus, the Japanese horseshoe crab. Journal of Biochemistry 93(6): 1539-1546, 1983

The aquatic vertebrate embryo as a sentinel for toxins: zebrafish embryo dechorionation and perivitelline space microinjection. International Journal of Developmental Biology 41(2): 411-423, 1997

Embryo and organ cultures of the horseshoe crab, Tachypleus tridentatus. Development Growth Diffn 164: 295-304, 1974

Swelling mechanism of the embryo of the japanese horseshoe crab tachypleus tridentatus. Zoological Magazine (Tokyo) 90(3): 271-282, 1981