Studies on the application of thin layer isoelectric focusing to food analysis 1. identification of fish species of sliced escolar lepidocybium flavobrunneum and castor oil fish ruvettus pretiosus
Ukishima, Y.; Narita, H.; Masui, T.; Nara, M.
Eisei Kagaku 30(4): 189-193
1984
Accession: 006513563
The sale of escolar (Lepidocybium flavobrunneum) and castor oil fish (Ruvettus pretiosus) is prohibited in Japan, because their muscles contain a large amount of wax and may cause food poisoning. However, it is difficult to distinguish them from edible fishes when they are sliced. Therefore, identification of these fishes by thin layer isoelectric focusing of sarcoplasmic proteins was attempted. The results showed that the electrophorograms of these proteins were species-specific, and therefore they were effectively utilized to distinguish these 2 fishes from other 26 edible fish species so far tested. In addition, seasoned slices of fishes were analyzed for sarcoplasmic proteins. The results obtained showed that the identification of each species was possible by thin layer isoelectric focusing of these sarcoplasmic proteins after desalting by gel filtration.