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Studies on the artificial reproduction of tricholoma matsutake 7. antibiotic activities of volatile substances extracted from a shiro of tricholoma matsutake



Studies on the artificial reproduction of tricholoma matsutake 7. antibiotic activities of volatile substances extracted from a shiro of tricholoma matsutake



Nippon Kingakukai Kaiho 20(2): 211-219



Volatile organic substances were extracted with steam distillation or ethylether from an active mycorrhiza of T. matsutake. The extracts, fractionated with gas chromatography, contained various volatile substances; 6 fractions (A-F) were main components. Components A-D were more abundant in the ether extracted fractions; component F was higher in the steam distillated fractions. These extracts suppressed growth of Bacillus subtilis in vitro; no effect on the growth of Penicillium citrinum or Trichoderma viride was noticeable. By comparison of retention times between each component and authentic terpenes, component A was probably .alpha.-pinene, component B was possibly .beta.-pinene and limonene was assumed to be contained in the extracts. .alpha.-Pinene had a strong antibiotic activity against B. subtilis and when added to soil, number of soil bacteria and fungi in the soil were reduced.

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Studies on the artificial reproduction of Tricholoma matsutake (S. Ito & Imai) Sing. VII. Antibiotic activities of volatile substances extracted from a Shiro of T. matsutake. Transactions of the Mycological Society of Japan 20(2): 211-219, 1979

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