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Studies on the fragmented shoot apex of grapevine vitis vinifera cultivar cabernet sauvignon 1. the regenerative capacity of leaf primordial fragments in vitro



Studies on the fragmented shoot apex of grapevine vitis vinifera cultivar cabernet sauvignon 1. the regenerative capacity of leaf primordial fragments in vitro



Journal of Experimental Botany 31(121): 483-488



Apical fragments of grapevine [cv. Cabernet Sauvignon] shoots cultured in a liquid medium containing cytokinin each develop into leaf-like structures which give rise to adventitious buds on transfer to solid medium. The origin of the leaf-like structures forming the 1st part of this procedure was examined. Microdissection of the shoot apex revealed that leaf primordial pieces regenerated into leaf-like structures in droplet culture. These leaves were regenerated directly from primordial cells without the need for dedifferentiation and callus formation. The implications of these results are discussed in relation to studies on leaf determination.

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Accession: 006516880

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