Studies on the function of pro plastids in the metabolism of in vitro cultured tobacco cells part 3 source of reducing power for amino acid synthesis from nitrite

Washitani, I.; Sato, S.

Plant and Cell Physiology 18(6): 1235-1242


ISSN/ISBN: 0032-0781
Accession: 006516956

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Evidence to show the presence of glucose-6-phosphate dehydrogenase [EC], 6-phosphogluconate dehydrogenase [EC], and NADP-dependent malic enzyme [EC] in proplastids of in vitro cultured tobacco [Nicotiana tabacum] cells was obtained. Amino acid synthesis from nitrite and 2-oxoglutarate in the proplastids was stimulated by the addition of 20 mM G-6-P. 6-Phosphogluconate, malate and isocitrate did not affect the synthesis. Nitrite reduction and glutamate synthesis in the proplastids are assumed to be supplied with NADPH2 as the source of reducing power through the reactions catalyzed by glucose-6-phosphate dehydrogenase and 6-phosphoglyconate dehydrogenase.