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Studies on the pathogenic fungus of mycotic granulomatosis part 3 development of the medium for mycotic granulomatosis fungus



Studies on the pathogenic fungus of mycotic granulomatosis part 3 development of the medium for mycotic granulomatosis fungus



Fish Pathology 13(3): 147-152



MG fungus, a causative agent of mycotic granulomatosis, was incubated at 25.degree. C on agar containing 10% fish meat extract (FME). It showed the best growth on FME agar containing 1% glucose. A medium consisting of glucose, 0.25% yeast extract and 1.5% agar (GY medium) was the best for the growth of MG fungus. The fungus grew better on the GY medium than on the FME agar, though it grew somewhat better on a modified FME agar containing 1% glucose than on the GY medium. The fungus could grow on an agar containing 0.25% yeast extract only, but could not grow on an agar with 1% glucose only. Apparently, a growth factor of the fungus existed in yeast extract. The fungus grows on neither Sabouraud dextrose agar nor Czapex dox agar. Culturing tests using FME agar containing the main intgredients of Sabouraud dextrose agar and those of Czapex dox agar revealed that a combination of 4% glucose and 1% peptone and that of 3% glucose and 0.1% K2HPO4 inhibit the fungus growth.

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