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Studies on the structure of reovirus cores selective removal of poly peptide lambda 2



Studies on the structure of reovirus cores selective removal of poly peptide lambda 2



Virology 70(1): 171-180



Exposure of reovirus cores or top component cores, to pH 11.8 at 4.degree. C removed nearly all of polypeptide .lambda.2. This effect occurred only within a narrow pH range; exposure to pH 11.4 resulted in less than 50% removal of .lambda.2 and exposure to pH 12.4 completely destroyed the cores. Exposure to pH 11.8 at 4.degree. C also removed the 12 spikes that are present on the surface of cores and reduced transcriptase activity by more than 90%. Iodination studies demonstrated that .lambda.1 was the more accessible of the 2 major polypeptides which remained in cores after alkali treatment.

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Accession: 006522115

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PMID: 3879



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