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Studies on the sucrose phosphate synthetase kinetic mechanism


Archives of Biochemistry and Biophysics 180(2): 298-302
Studies on the sucrose phosphate synthetase kinetic mechanism
The kinetic properties of wheat germ sucrose phosphatase synthetase, which catalyzes the reaction UDP-glucose + fructose 6-phosphate .fwdarw. UDP + sucrose 6-phosphate were studied. A plot of the reciprocal initial velocity vs. reciprocal substrate concentration gave a series of intersecting lines indicating a sequential mechanism. Product inhibition studies showed that UDP was competitive with UDP-glucose and noncompetitive with fructose 6-phosphate. A dead-end inhibitor, Pi, was competitive with UDP-glucose and noncompetitive with fructose 6-phosphate. The results of initial velocity and product and dead-end inhibition studies suggested that the addition of substrates to the enzyme follows an ordered mechanism.

Accession: 006522186

PMID: 879791

DOI: 10.1016/0003-9861(77)90041-8

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