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Studies on the temperate bacteriophages from bacillus licheniformis ii. molecular biological characterization of dna

Studies on the temperate bacteriophages from bacillus licheniformis ii. molecular biological characterization of dna

Acta Genetica Sinica 13(3): 179-183

DNA was extracted from a group of phages: Blp7, Blp11, and Blp16. By using electron microscopic measurement, the contour length of Blp7 DNA was determined to be 62.75 .+-. 1.47 kb. These phages contained a cos site, and the similarity between them and .lambda. phage was identified. After DNA partial denaturation with alkaline, the R- end or L-end of Blp7 DNA was recognized under the electron microscope. Blp7 and Blp16 DNA were digested with restriction endonuclease EcoRI and BamHI, and the DNA polymorphism was observed.

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