EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Studies on the temperate bacteriophages from bacillus licheniformis ii. molecular biological characterization of dna






Acta Genetica Sinica 13(3): 179-183

Studies on the temperate bacteriophages from bacillus licheniformis ii. molecular biological characterization of dna

DNA was extracted from a group of phages: Blp7, Blp11, and Blp16. By using electron microscopic measurement, the contour length of Blp7 DNA was determined to be 62.75 .+-. 1.47 kb. These phages contained a cos site, and the similarity between them and .lambda. phage was identified. After DNA partial denaturation with alkaline, the R- end or L-end of Blp7 DNA was recognized under the electron microscope. Blp7 and Blp16 DNA were digested with restriction endonuclease EcoRI and BamHI, and the DNA polymorphism was observed.


Accession: 006522428



Related references

Li, Y.; Chen, Y.; Chu, Z., 1986: Studies on the temperate bacteriophages from bacillus licheniformis i. biological characterization. Seven temperate bacteriophages were isolated from different Bacillus licheniformis strains. The morphological character of plaque and particle, the stability and immunity of lysogen, the serological specificity of these phages were studied. Accord...

Wilson, G.A.; Williams, M.T.; Baney, H.W.; Young, F.E., 1974: Characterization of temperate bacteriophages of Bacillus subtilis by the restriction endonuclease EcoRI: evidence for three different temperate bacteriophages. Temperate bacteriophages of Bacillus subtilis were characterized according to host range and digestion of the bacteriophage genome by endonuclease EcoRI. The three bacteriophages, phi3T, SPO2, and phi105, were all heteroimmune, and the DNA digests...

Huang, W.; Marmur, J., 1970: Characterization of inducible bacteriophages in Bacillus licheniformis. Two morphologically distinct and physically separable defective phages have been found in Bacillus licheniformis NRS 243 after induction by mitomycin C. One of them (PBLB) is similar to the defective phage PBSX of B. subtilis, which has a density...

Colasito, D.J.; Rogoff, M.H., 1969: Characterization of temperate bacteriophages of Bacillus thuringiensis. Journal of General Virology 5(2): 275-281

Relano, P.; Mata, M.; Bonneau, M.; Ritzenthaler, P., 1987: Molecular characterization and comparison of 38 virulent and temperate bacteriophages of Streptococcus lactis. Thirty-three virulent and five temperate phages of Streptococcus lactis and Streptococcus cremoris were differentiated into three groups by DNA homology. A complete lack of DNA homology was demonstrated between the phage groups. Within each group,...

Williams, M.T.; Young, F.E., 1977: Temperate Bacillus subtilis bacteriophage phi 3T: chromosomal attachment site and comparison with temperate bacteriophages phi 105 and SPO2. The temperate Bacillus subtilis bacteriophage phi 3T contains within its genome a locus, designated thyP3, that encodes for a protein with thymidylate synthetase activity. Bacteriophage phi 3T is different from the two previously characterized tem...

Mostafa, S.A.; Mahmoud, M.S.; Mohamed, Z.K.; Enan, M.R., 2009: Cloning and molecular characterization of chitinase from Bacillus licheniformis MS-3. Journal of General and Applied Microbiology 55(3): 241-246

Liang, W-Chi.; Wang, X-Hui.; Lin, M-Guan.; Lin, L-Liu., 2009: A 70-kDa molecular chaperone, DnaK, from the industrial bacterium Bacillus licheniformis: gene cloning, purification and molecular characterization of the recombinant protein. The heat shock protein 70 (Hsp70/DnaK) gene of Bacillus licheniformis is 1,839 bp in length encoding a polypeptide of 612 amino acid residues. The deduced amino acid sequence of the gene shares high sequence identity with other Hsp70/DnaK proteins...

Imanaka, T.; Uchida, K.; Tateishi, M.; Aiba, S., 1979: Inducible bacteriophages of Bacillus licheniformis ATCC 10716. Two inducible phages, LP52 and DLP 10716, were isolated from Bacillus licheniformis ATCC 10716. Molecular weights of DNAs of LP52 and DLP 10716 are 38.7 x 10(6) and 8.1 x 10(6), respectively. Restriction endonuclease EcoRI digested LP52 DNA and DL...

Friedman, S.O.; Hemphill, H.E., 1976: Genetic and physiological studies of abortive infections of hydroxymethyluracil-containing bacteriophages in lysogens of temperate Bacillus subtilis bacteriophage SPO2. Wild-type bacteriophage phie and IS (interference-sensitive) mutants of the related phage SP82G did not productively infect strains of Bacillus subtilis that were lysogenic for temperate phage SPO2. In these abortive infections, the sensitive phag...