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Studies on the template activity of isolated xenopus erythrocyte nuclei part 2 the effects of cytoplasmic extracts


Journal of Cell Science 24(1): 119-130
Studies on the template activity of isolated xenopus erythrocyte nuclei part 2 the effects of cytoplasmic extracts
The effects of exposing nuclei isolated from Xenopus erythrocytes to cytoplasmic extracts prepared from various sources on their template activity are described. The cytoplasm of rat liver cells and the immature blood cells of Xenopus contain factors which stimulate RNA synthesis in these essentially inactive nuclei, whereas the cytoplasm of many other cells does not yield such factors. The active factors in rat liver cytoplasmic extracts appear to be proteins with a MW of about 40,000 daltons. The implications for genetic control in eucaryotes are discussed.

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Accession: 006522437



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