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Studies on the tissue culture of Stevia rebaudiana and its components; (II). Induction of shoot primordia

Planta Medica: 321-323

Studies on the tissue culture of Stevia rebaudiana and its components; (II). Induction of shoot primordia

Shoot primordia, which were able to propagate vegetatively with a very high rate and to redifferentiate easily to new plants, were induced from shoot tips of Stevia rebaudiana Bertoni on Gamborg B5 medium containing 6-benzylaminopurine (BAP) and .alpha.-naphthaleneacetic acid (NAA) under light. The propagation of the shoot primordia of Stevia rebaudiana is rapid, and they are highly stable in chromosome number and karyotype. The shoot primordia can propagate at a high rate for a long time without differentiation. At any time, the shoot primordia readily developed into plantlets with shoots and roots within 2 or 3 weeks in static culture on B5 medium containing 0.02 mg/l BAP and 2% sucrose. The plantlets were transplanted to sterilized soil to grow to normal adult plants.

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Accession: 006522516

PMID: 17345320

DOI: 10.1055/s-2007-969165

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