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Studies on the tissue culture of Stevia rebaudiana and its components; (II). Induction of shoot primordia

, : Studies on the tissue culture of Stevia rebaudiana and its components; (II). Induction of shoot primordia. Planta Medica: 321-323

Shoot primordia, which were able to propagate vegetatively with a very high rate and to redifferentiate easily to new plants, were induced from shoot tips of Stevia rebaudiana Bertoni on Gamborg B5 medium containing 6-benzylaminopurine (BAP) and .alpha.-naphthaleneacetic acid (NAA) under light. The propagation of the shoot primordia of Stevia rebaudiana is rapid, and they are highly stable in chromosome number and karyotype. The shoot primordia can propagate at a high rate for a long time without differentiation. At any time, the shoot primordia readily developed into plantlets with shoots and roots within 2 or 3 weeks in static culture on B5 medium containing 0.02 mg/l BAP and 2% sucrose. The plantlets were transplanted to sterilized soil to grow to normal adult plants.

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Accession: 006522516

PMID: 17345320

DOI: 10.1055/s-2007-969165

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Related references

Miyagawa, H.F.jioka, N.K.hda, H.Y.masaki, K.T.nguchi, K.T.naka, R., 1986: Studies on the tissue culture of Stevia rebaudiana and its components. II. Induction of shoot primoridia. Planta medica = journal of medicinal plant research: (4) 321-323

Miyagawa H.; Fujita Y.; Fujioka N.; Kohda H.; Yamasaki K., 1984: Studies on the tissue culture of stevia rebaudiana and its components 1. Callus was induced from stem segments and shoot tips of S. rebaudiana Bertoni on Murashige and Skoog (MS) medium containing 2,4-D (10-7-10-5 M) and kinetin (0-10-5 M) in the dark or light. The growth rate of callus culture increased as the concent...

Lee C.W.; Pak C H.; Hughes H.G., 1990: Tissue culture studies on stevia rebaudiana as a source of new sweetener crop. Hortscience 25(9): 1109

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Lee, J.I.; Kang, K.H.; Park, H.W.; Ham, Y.S.; Park, C.H., 1980: Studies on the new sweetening source plant, Stevia rebaudiana in Korea. II. Effects of fertilizer rates and planting density on dry leaf yields and various agronomic characteristics of Stevia rebaudiana. Nitrogen P2O5 and K2O at rates of 0, 10 and 15 kg/10 a each, applied in various combinations did not affect Stevia rebaudiana plant height, number of branches or number of internodes, but the dry leaf yield was best when 10 kg of each nutrient was...

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Prakash, I.; Bunders, C.; Devkota, K.P.; Charan, R.D.; Ramirez, C.; Priedemann, C.; Markosyan, A., 2014: Isolation and characterization of a novel rebaudioside M isomer from a bioconversion reaction of rebaudioside A and NMR comparison studies of rebaudioside M isolated from Stevia rebaudiana Bertoni and Stevia rebaudiana Morita. A minor product, rebaudioside M2 (2), from the bioconversion reaction of rebaudioside A (4) to rebaudioside D (3), was isolated and the complete structure of the novel steviol glycoside was determined. Rebaudioside M2 (2) is considered an isomer o...

Shi, H.J.; Gong, H.Y., 1981: Preliminary report on tissue culture in Stevia rebaudiana Hemsl. Whole plantlets were regenerated from leaf segments in vitro.