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Studies on the toluene 2 4 diisocyanate induced shape change of human erythrocytes

Studies on the toluene 2 4 diisocyanate induced shape change of human erythrocytes

Journal of Tokyo Women's Medical College 54(7): 581-594

The biconcave discoid shape of normal human erythrocytes can be altered into cup-shaped cells by bifunctional reagent toluene 2,4-diisocyanate (TDI). The extent of TDI-induced shape change of the erythrocytes seems to be correlative to the concentration of TDI. The resultant modified red cells were in progressive decrease in the filtrability as measured by microporous membrane and also an increase in resistance to hemolysis against water. Isolated membranes obtained from TDI-treated cells showed cross-linking of membrane proteins and phospholipids, mainly of spectrin, phosphatidylethanolamine and phosphatidylserine, during shape change in different degree according to the concentration of TDI. Isolated erythrocyte ghosts underwent TDI-dependent shape that was similar to intact cells. After treating control ghosts with trypsin, some bands, including spectrin, band 2.1 and 4.1 disappeared concomitantly on polyacrylamide gel electrophoresis. Apparently, membrane proteins, cytoskeletal network underlying erythrocyte membranes, are necessary for maintaining and changing the shape of red cells.

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