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Studies on the usefulness of the human basophil degranulation test made by an allergolam kit



Studies on the usefulness of the human basophil degranulation test made by an allergolam kit



Japanese Journal of Allergology 35(12): 1139-1148



It is known that degranulation and the release of chemical mediators such as histamine from human basophils can be observed when the cells are challenged by allergens. Basophils usually occupy less than 1% of peripheral leucocytes. A method for obtaining a basophil-rich fraction by the addition of mixture of sodium metrizoate and ficoll to peripheral blood followed by centrifugation has been established by Dry et al. The Pasteur Institute (France) has produced the "Allergolam" a human basophil degranulation test (HBDT) kit, for the identification of allergens. It works by calculating the decrease in the number of stainable basophils after the reaction of a basophil-rich fraction in wells coated with allergens. We studied the usefulness of the kit using 202 blood samples from normal subjects and atopic patients in 5 institutes. The correlation between the results of HBDT and RAST was 84.3%, 83.8% and 73.0% in the case of house dust (HD), mite and a mixture of cat- and dog hair (CD) respectively. The correlation between the results of HBDT and skin test was 70.1%, 68.3%, 68.5% and 76.9% in the case of HD, mite, CD and a mixture of mold and fungi, respectively. The correlation coefficient between HBDT and histamine release test was 0.81 and 0.73 in the case of HD and mite respectively. Therefore, HBDT using these kits was thought to be usefeul for the identification of allergens and for studies of allergic reactions.

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