Studies on the utilization of pasture herbage nitrogen by rumen microorganisms 3. effects of carbohydrate and lipid material on the release of ammonia from the herbage nitrogen constituents and microbial ammonia incorporation in in vitro incubation with nitrogen 15 ammonia tracing technique

Tano, H.; Shibata, F.

Grassland Science 32(1): 66-71

1986


ISSN/ISBN: 1744-6961
Accession: 006522881

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Abstract
The present experiment was undertaken with a 15N ammonia tracing technique to examine the effects of various carbohydrates and two kinds of lipid materials on the release of ammonia from the grass nitrogen constituents by rumen microorganisms and on the microbial ammonia incorporation simultaneously occurring, under the condition of non-steady-state of ruminal ammonia concentration in vitro. The strained rumen fluid, obtained from a goat fed on alfalfa hay, was incubated with freeze-dried Italian ryegrass and (15NH4)2SO4 solution for 90 min. Compared with control incubation incubation (without additives), the addition of all of carbohydrates used and corn oil did not vary the quantity of ammonia release from the grass nitrogen. Corn 'oil foots' exceptionally lowered it. The effects of carbohydrates and lipid materials on the microbial ammonia incorporation were as follows: In the case of polysaccharides, xylan and inulin increased appreciably the microbial ammonia incorporation. Xylan was the most effective one. Cellulose showed no effect while corn starch and pectin showed a little effect. Among the disaccharides used, cellobiose was superior to maltose while sucrose and trehalose showed a little effect. Among the monosaccharides supplied, galactose, arabinose and xylose were more effective than glucose, mannose and fructose. In the two lipid materials, corn oil had no effect while corn 'oil foots' tended to lower the microbial ammonia incorporation. From these results, it was concluded that the certain carbohydrate which was effective on lowering the ruminal ammonia production was not functioning as a depressor of the ammonia release from grass nitrogen by rumen microorganisms but was functioning as a stimulator of microbial ammonia incorporation simultaneously occurring. On the effect of corn 'oil foots', it was indicated that the material not only depressed the ammonia release but also lowered the microbial ammonia incorporation.