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Study in sporotrichosis iii. histological and immunohistochemical study in experimental cutaneous sporotrichosis in man



Study in sporotrichosis iii. histological and immunohistochemical study in experimental cutaneous sporotrichosis in man



Yokohama Medical Bulletin 38(1-2): 37-48



The defence mechanism and the influence of corticosteroid application were studied in experimental sporotrichosis in man. A healthy adult man was intracutaneously injected Sporothrix schenckii spore suspension (1.8 .times. 106 spores) at 12 sites on his forearm. A half of the sites were applied corticosteroid ointment three times daily. The injected sites were successively biopsied on day 3, 7, 14, 21 and 28. The specimens ere examined histopathologically and immunohistochemically using PAP method (antibodies used were; anti-MT-1, IgG, IgA, IgM, IgE, C3, lysozyme, .alpha.1-antitrypsin, .alpha.1-antichymotrypsin and S-100 protein). The specimen taken on day 28 was also examined using ABC method (antibodies used were; anti-Leu1, 2a, 3a, 4, 6, 7, 14, M1 and anti-OKIa1). Histopathologically, control sites showed in its course abscess formation, transepidermal elimination of the abscess, maturation of Mo-M.vphi. around the abscess, and increase of the lymphocytes surrounding the granuloma. Fungal elements were seen mostly in the granuloma, but gradually decreased during the course. Corticosteroid inhibited these processes, and in steroid-applied group the number of fungal elements increased during the course to form a mass of innumerable spores seen in the specimen taken on day 28. Immunohistochemically, PMNs, T cells, B cells, plasma cells (IgG-, IgA- and IgM-positive), histiocytes and giant cells were identified. IgE-, S-100 protein-positive cells, NK cells, Langerhans cells and C3-positive cells were not detected. The subpopulations in both control and steroid-applied groups were the same in essential. From these results it is assumed that PMN, M.vphi., T and B cells contribute and interact in the defence mechanism of sporotrichosis. It is also shown that steroid application facilitated the fungal proliferation, probably via the inhibition of PMN aggregation, which clinically give rise to sporotrichosis with numerous fungal elements.

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