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Study of adsorption and other side effects on guar gel filtration media and preparation and applications of guar gel ion exchangers

Study of adsorption and other side effects on guar gel filtration media and preparation and applications of guar gel ion exchangers

Journal of Chromatography 177(2): 209-218

The elution behavior of low MW substances such as common buffering substances, amino acids and some aromatic and heterocyclic compounds on guar 5-X30 gel was investigated. Gel filtration behavior was determined by measuring distribution coefficients. Among inorganic substances, hydroxyl and borate ions were strongly adsorbed on the gel matrix. Among organic compounds, aromatic and heterocyclic substances show a greater tendency than aliphatic substances to be adsorbed. Basic groups in the test substances tend to increase adsorption, while acidic groups have the opposite effect. A cation exchanger (CM-guar 5-X30) and an anion exchanger (DEAE-guar 5-X30) were synthesized by reaction of guar 5-X30 gel with monochloroacetic acid and 1-chloro-2-diethylaminoethane hydrochloride, respectively, in a strongly alkaline medium. Efficiency of CM-guar 5-X30 and DEAE-guar 5-X30 as packings for ion-exchange chromatography was demonstrated.

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