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Study of transfer rna structure by energy transfer method using fluorescent probes covalently attached to specific sites of transfer rna

Study of transfer rna structure by energy transfer method using fluorescent probes covalently attached to specific sites of transfer rna

Molekulyarnaya Biologiya (Moscow) 12(2): 385-396

Optical and fluorescent characteristics of fluorescein covalently attached to 3'-end of tRNAPhe and X-nucleotide in the extra arm of several species of tRNA from Escherichia coli were studied. The probe is a sensitive factor indicating the conformational change of tRNA induced by Mg2+ and Na+ ions. Measuring the extent of energy transfer showed that the distances between the fluorescent probe attached to 3'-terminus and X-nucleotide of tRNA and specific binding site of ethidium bromide on tRNA were 40.5 .ANG. and 32.5 .ANG., respectively. The distances measured are in good agreement with the NMR spectroscopy data showing that the specific binding site for ethidium bromide on tRNA is localized near the 6th base pair of the acceptor stem.

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Accession: 006529927

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