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Study of urinary trypsin inhibitor i. fundamental investigation of radioimmunoassay of urinary trypsin inhibitor



Study of urinary trypsin inhibitor i. fundamental investigation of radioimmunoassay of urinary trypsin inhibitor



Medical Journal of Hiroshima University 34(6): 849-856



For clinical application of measurement of urinary trypsin inhibitor with radioimmunoassay method (UTI-RIA), the fundamental study for this method was performed. The results obtained are summarized as follows: 1) The influences of the method and the term of the storage of urine specimens on UTI-RIA were studied. The value of UTI estimated at the several conditions of urine specimen except for dialysis was changed insignificantly in comparison with that of fresh urine. From these results, it was not necessary to prepare the storage of urine samples prior to UTI-RIA. In addition, on the condition of the storage under 4.degree. C, the value of UTI was stabilized for 2 months. 2) The daily change and error of the UTI excretion was investigated in 10 healthy men. On the daily change, the UTI excretion increased in the morning, and then slightly decreased at the daytime, however, UTI excretion during night significantly decreased in comparison with that in the morning (p < 0.05). The daily error of UTI excretion was insignificantly changed. 3) On the influence of hematuria, the value fo UTI in the urine added blood was compared to that in the clear urine. UTI-RIA was not influenced with hematuria less than 0.2%. 4) On the influence of infected urine, the UTI value in clear urine was compared with that in infected urine in the patients with benign prostatic hyperplasia. It was shown that the UTI value changed insignificantly between two groups, suggesting that the UTI value was not influenced by the infected urine. From the above-mentioned results, it was obtained that UTI-RIA was able to use clinical application.

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