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Study on plasmids coding for enterotoxin 4. characterization and detection of colonization factor antigen i and ii cfa i and cfa ii in enterotoxigenic escherichia coli isolated from humans



Study on plasmids coding for enterotoxin 4. characterization and detection of colonization factor antigen i and ii cfa i and cfa ii in enterotoxigenic escherichia coli isolated from humans



Zhonghua Weishengwuxue He Mianyixue Zazhi 7(4): 230-233



Colonization factor antigen I and II (CFA/I and CFA/II) play an important role in the pathogenesis of diarrhea due to enterotoxigenic Escherichia coli. In this study, ETEC strains isolated from 130 persons with diarrhea and E. coli strains isolated from 269 healthy neonates were examined for the presence of CFA/I and CFA/II, using mannose-resistant hemagglutination, salt aggregation, bacterial agglutination with CFA/I and CFA/II antisera, indirect fluorescent microscopy, electron microscopy and immune electron microscopy. CFA/I and CFA/II were identified in 13 of 34 strains (38%) with heat-labile and heat-stable enterotoxins (LT+/ST+), but in only 4 of 35 LT-/ST+ strains (11%, P < 0.01) and 5 of 61 LT+/ST- strains (8%, P < 0.01). CFA/I and CFA/II were not found among 269 non-ETEC strains. The ETEC strains bearing a CFA/I and CFA/II had some properties: 1) showing MRHAhum or MRHAbov; 2) the lowest (NH4)2SO4 concentration that caused bacterial aggregation being 0.2 molarity; 3) electron micrographs of bacteria showing CFA/I or CFA/II fimbriae on their surface and CFA/I or CFA/II antiserum absorbed to the surface associated filaments.

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