Sub cellular localization of flavonoid synthesizing enzymes in pisum sativum cultivar midfreezer phaseolus vulgaris cultivar early gallatin brassica oleracea cultivar red danish and spinacia oleracea cultivar bloomsdale cultivars
Hrazdina, G.; Alscher Herman, R.; Kish, V.M.
Phytochemistry 19(7): 1355-1360
ISSN/ISBN: 0031-9422 Accession: 006532460
Functionally-intact chloroplasts were obtained from 11-day-old pea (Pisum sativum cv. Midfreezer) seedlings. Enzyme distribution studies with ribulose bisphosphate carboxylase and NADPH-dependent glyceraldehyde-3-phosphate dehydrogenase showed that approximately 2.1% of the total tissue chloroplasts were present in the chloroplast preparation. The rate of intactness of chloroplast preparations was 34-82%. SAM(S-adenosyl-L-methionine):caffeic acid methyltransferase, flavanone synthase, UDPG(uridine diphosphate glucose):flavonoid-3-O-glucosyltransferase and SAM:quercetin methyltransferase activities were measured in the homogenate, supernatant and chloroplast lysate fractions. Significant activities of the above 4 enzymes could only be detected in the homogenate and supernatant fractions. Similar experiments with 11-day-old seedlings of green beans (Phaseolus vulgaris cv. Early Gallatin), red cabbage (B. oleracea cv. Red Danish) and 6-wk-old plants of spinach (S. oleracea cv. Bloomsdale) showed a similar distribution of the flavonoid synthesizing enzymes. Under the reported conditions, chloroplasts were not involved in flavonoid biosynthesis.