Section 7
Chapter 6,533

Sub cellular localization of flavonoid synthesizing enzymes in pisum sativum cultivar midfreezer phaseolus vulgaris cultivar early gallatin brassica oleracea cultivar red danish and spinacia oleracea cultivar bloomsdale cultivars

Hrazdina, G.; Alscher Herman, R.; Kish, V.M.

Phytochemistry 19(7): 1355-1360


ISSN/ISBN: 0031-9422
Accession: 006532460

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Functionally-intact chloroplasts were obtained from 11-day-old pea (Pisum sativum cv. Midfreezer) seedlings. Enzyme distribution studies with ribulose bisphosphate carboxylase and NADPH-dependent glyceraldehyde-3-phosphate dehydrogenase showed that approximately 2.1% of the total tissue chloroplasts were present in the chloroplast preparation. The rate of intactness of chloroplast preparations was 34-82%. SAM(S-adenosyl-L-methionine):caffeic acid methyltransferase, flavanone synthase, UDPG(uridine diphosphate glucose):flavonoid-3-O-glucosyltransferase and SAM:quercetin methyltransferase activities were measured in the homogenate, supernatant and chloroplast lysate fractions. Significant activities of the above 4 enzymes could only be detected in the homogenate and supernatant fractions. Similar experiments with 11-day-old seedlings of green beans (Phaseolus vulgaris cv. Early Gallatin), red cabbage (B. oleracea cv. Red Danish) and 6-wk-old plants of spinach (S. oleracea cv. Bloomsdale) showed a similar distribution of the flavonoid synthesizing enzymes. Under the reported conditions, chloroplasts were not involved in flavonoid biosynthesis.

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