+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Substrate specificity of calcium cam dependent multifunctional protein kinases comparison of isoenzymes from brain liver and skeletal muscle



Substrate specificity of calcium cam dependent multifunctional protein kinases comparison of isoenzymes from brain liver and skeletal muscle



Biochemical & Biophysical Research Communications 151(3): 1332-1338



Ca2+/CaM-dependent multifunctional protein kinase isoenzymes from brain, skeletal muscle and liver were compared by their phosphorylation of a number of protein substrates. Under the conditions of assay, the three isoenzymes demonstrated rapid phosphorylation of synapsin I and glycogen synthase. In contrast, rates of phosphorylation of pyruvate kinase and phenylalanine hydroxylase were almost two orders of magnitude slower. Differences in phosphorylation specifically of the latter two substrates was also observed among the three protein kinases. Phosphorylation by Ca2+/CaM-dependent protein kinases was contrasted with cAMP-dependent protein kinase, which phosphorylates these proteins in vitro and in vivo. The potential role of Ca2+/CaM-dependent multifunctional protein kinases in the Ca2+-dependent phosphorylation of these substrates is discussed.

(PDF emailed within 1 workday: $29.90)

Accession: 006536571

Download citation: RISBibTeXText


Related references

Substrate specificity of Ca2+/CaM-dependent multifunctional protein kinases: comparison of isoenzymes from brain, liver and skeletal muscle. Biochemical and Biophysical Research Communications 151(3): 1332-1338, 1988

Purification of the multifunctional calmodulin-dependent protein kinases from lung and liver, and the comparison to the brain enzyme. The Japanese Journal of Pharmacology 46(2): 173-182, 1988

Calcium calmodulin-dependent microtubule-associated protein 2 kinase: broad substrate specificity and multifunctional potential in diverse tissues. Biochemistry 24(20): 5320-5327, 1985

Calcium-dependent protein kinases from Arabidopsis show substrate specificity differences in an analysis of 103 substrates. Frontiers in Plant Science 2: 36, 2011

Skeletal muscle sarcolemma proteins as targets for adenosine 3':5'-monophosphate-dependent and calcium-dependent protein kinases. Archives of Biochemistry and Biophysics 262(1): 245-258, 1988

Cyclic AMP-dependent protein kinases from skeletal muscle and liver. Advances in Cyclic Nucleotide Research 1: 33-45, 1972

The substrate specificity of cyclic amp dependent protein kinase of rabbit skeletal muscle. Biochemical Journal 162(2): 411-422, 1977

Phosphorylation of multiple sites in a 15 000 dalton proteolipid from rat skeletal muscle sarcolemma, catalyzed by adenosine 3,5-monophosphate-dependent and calcium / phospholipid-dependent protein kinases. Biochimica et Biophysica Acta - Molecular Cell Research 968(1): 127-137, 1988

Phosphorylation of multiple sites in a 15,000 dalton proteolipid from rat skeletal muscle sarcolemma, catalyzed by adenosine 3',5'-monophosphate-dependent and calcium/phospholipid-dependent protein kinases. Biochimica et Biophysica Acta 968(1): 127-137, 1988

The substrate specificity of adenosine 3':5'-cyclic monophosphate-dependent protein kinase of rabbit skeletal muscle. Biochemical Journal 162(2): 411-421, 1977

Substrate specificity of rat brain calcium-activated and phospholipid-dependent protein kinase. Biochemical and Biophysical Research Communications 134(3): 1358-1364, 1986

Endogenous substrate proteins for calcium calmodulin dependent calcium phospho lipid dependent and cyclic amp dependent protein kinases in mouse pancreatic islets. Biochemical Journal 221(1): 247-254, 1984

Comparison of adenosine 3':5'-monophosphate-dependent protein kinases from rabbit skeletal and bovine heart muscle. Journal of Biological Chemistry 250(19): 7795-7801, 1975

Substrate specificity of a multifunctional calmodulin-dependent protein kinase. Journal of Biological Chemistry 260(27): 14471-6, 1985

Existence of endogenous substrate proteins for calcium calmodulin dependent and calcium phospholipid dependent protein kinases in rat adrenal glomerulosa cells. Journal of Steroid Biochemistry 29(3): 277-284, 1988