Subunit structure of phenol oxidase purified from the larvae of house fly musca domestica vicina

Yamaura, I.; Yonekura, M.; Katsura, Y.; Ishiguro, M.; Funatsu, M.

Agricultural and Biological Chemistry 44(10): 2437-2441


ISSN/ISBN: 0002-1369
Accession: 006537465

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The subunit structure of phenol oxidase from the larvae of housefly, M. domestica vicina Maquart, was investigated by urea treatment, SDS-polyacrylamide gel electrophoresis and EM. Phenol oxidase was dissociated into a single type of subunit (MW 6.5 .times. 104) by 5 M urea treatment. A similar subunit (MW 6.1 .times. 104) was detected by SDS-polyacrylamide gel electrophoresis. Additional protein bands (4) with MW of 4.8 .times. 104, 3.5 .times. 104, 2.4 .times. 104 and 1.2 .times. 104 were detected by SDS-polyacrylamide gel electrophoresis by extensive incubation of phenol oxidase with SDS. A cylindrical molecular structure was deduced from the EM analysis. The outside and inside diameter and the height of phenol oxidase molecule were evaluated to be 100, 50 and 70 .ANG. respectively.