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Surface activity of various natural surfactant preparations and their effects on lung thorax pressure volume characteristics of premature newborn rabbits comparison with surfactant ta

Surface activity of various natural surfactant preparations and their effects on lung thorax pressure volume characteristics of premature newborn rabbits comparison with surfactant ta

Journal of the Iwate Medical Association 37(4): 563-574

The purpose of this study was to compare in vitro and in vivo surface activities of various natural surfactant preparations with Surfactant TA (Tokyo-Akita, S-TA). Crude natural surfactant (CNS, n = 10) was obtained from lung lavage of 10 bovines by differential centrifugations. CNS lipid (CNS-L, n = 10) was obtained from CNS by the Folch's procedure and purified natural surfactant (PNS, n = 10) was obtained by the Shelley's procedure. Surface spreading and adsorption characteristics, surface-tension lowering ability and surface compressibility of these surfactant preparations were measured by modified Wilhelmy surface balance at C. S-TA satisfied all the criteria for optimal surfactant. Both CNS-L and PNS had the two major functional requirements of pulmonary surfactant, i.e., rapid spontaneous surface spreading and adsorption characteristics, but did not satisfy all the criteria for optimal surfactant. CNS did not meet any of these criteria. Rabbit fetuses were delivered by hysterotomy on day 27 (.+-. 3 hrs) of gestation. Before first breath, they were killed by exanguination via transabdominal dissection of the aorta. Each animal was sealed into a separate compartment of a multichamber, whole body-plethysmograph heated to C. 50 .mu.l of surfactant dispersion (33 mg phospholipid/ml) was instilled into the trachea; 38 fetuses received S-TA, 79 received CNS, 73 received CNS-L, 78 received PNS and 38 littermates served as controls. PV characteristics of the lung-thorax of these animals were measured. The lung volume (LV) per kg of body weight on initial deflation was used for comparison of PV of each surfactant. LV at each pressure was significantly higher in the group S-TA than the rest of groups. Coefficients of variation were considerably high in the groups CNS, CNS-L, and PNS as compared with the group S-TA. It is concluded that a large batch-to-batch variability with inferior surface activity of natural surfactant preparations decreases the possibility of their application in the treatment of RDS in preterm infants.

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Accession: 006546428

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