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Surface areas of lipid membranes

Surface areas of lipid membranes

Biochemistry 17(5): 837-840

Upon photolysis, alkyl pentacyanocobaltate complexes generate alkyl radicals which react rapidly and specifically with nitroxide radicals and which do not penetrate phospholipid bilayers. By measuring the loss of paramagnetic resonance signal intensity when multilamellar liposomes containing a small amount of spin-labeled lipid are exposed to these radicals, the proportion of lipid on the external surface of liposomes was measured. Liposomes prepared under specified conditions from dimyristoylphosphatidylcholine, dipalmitoylphosphatidylcholine and binary mixtures of dipalmitoylphosphatidylcholine and cholesterol all have the same proportion of external lipid.

Accession: 006546605

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PMID: 629935

DOI: 10.1021/bi00598a014

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