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Surface charge of membrane erythrocytes with disturbances of lipid metabolism from the data of microelectrophoresis proton titration and fluorescence investigations






Ukrainskii Biokhimicheskii Zhurnal 60(1): 74-84

Surface charge of membrane erythrocytes with disturbances of lipid metabolism from the data of microelectrophoresis proton titration and fluorescence investigations

The surface charges and the isoelectric points (pI) as well as changes in these values depending on the lipid composition are determined for erythrocyte membranes of rat, rabbit, bull and man under conditions of hypercholesterinemia, atherosclerosis, D-hypovitaminosis and in experiments in vitro using the techniques of microelectrophoresis and positive charged ligands (proton, probe astraphloxin) binding. The ionogenic groups forming charge and essential differences in their nature (pK) and quantity depending on the erythrocyte type are found. Inverse correlation between the content of cholesterol and cholesterol ethers and the value of erythrocyte membrane surface charges was established for each sample examined. A comparative evaluation of the methods used was conducted.

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Accession: 006546750



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